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New England Biolabs
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Addgene inc
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Addgene inc
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Addgene inc
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Shanghai GenePharma
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Ribobio co
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Addgene inc
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Addgene inc
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Addgene inc
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Addgene inc
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Addgene inc
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Addgene inc
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Image Search Results
Journal: Current issues in molecular biology
Article Title: Identification and Characterization of Cancer Stem-Like Cells in ALK-Positive Anaplastic Large Cell Lymphoma Using the SORE6 Reporter.
doi: 10.3390/cimb43020041
Figure Lengend Snippet: Figure 3. Experimental manipulation of Sox2 and Oct4 in SupM2 SORE6 clones. (A) FACS analysis showing the effect on SORE6 reporter activity of overexpressing Sox2 in single-cell clone 5-4 (SupM2 SORE6−). (B) FACS analysis showing the effect on SORE6 reporter activity of overexpressing shSox2 in single-cell clone 6-5 (SupM2 SORE6+). (C) FACS analysis showing the effect of Oct4 downregulation on SORE6 reporter activity in clone 5-4 (SupM2 SORE6−). (D) FACS analysis showing the effect of shOct4 transfection on SORE6 reporter activity in single-cell clone 6-5 (SupM2 SORE6+). All results shown are representative of three independent experiments and were also repeated in a second batch of single-cell clones. Side panels show the fold change of GFP in log scale (mean ± SEM). Bottom panels of each figure are the Western blots showing the effect of transient transfection with Sox2, Oct4, shSox2, and shOct4 plasmids. Empty vector (EV) was included as a negative control. ** p < 0.01, *** p < 0.001.
Article Snippet: The Oct4 expression vector (pLVEF1a-hOCT4-IRES-Neo) was purchased from BiOSETTIA (San Diego, CA, USA).
Techniques: Clone Assay, Activity Assay, Transfection, Western Blot, Plasmid Preparation, Negative Control
Journal: Current issues in molecular biology
Article Title: Identification and Characterization of Cancer Stem-Like Cells in ALK-Positive Anaplastic Large Cell Lymphoma Using the SORE6 Reporter.
doi: 10.3390/cimb43020041
Figure Lengend Snippet: Figure 6. SORE6−and SORE6+ clones are biochemically distinct. (A) The subcellular localization of Sox2, Oct4, and c-Myc in SORE6−and SORE6+ cells derived from SupM2, assessed by the nuclear cytoplasmic fractionation assay. (B) The DNA pull-down assay was performed to assess Sox2, Oct4, and c-Myc transcriptional activity in SORE6−and SORE6+ cells using a biotin-labeled SORE6 probe.
Article Snippet: The Oct4 expression vector (pLVEF1a-hOCT4-IRES-Neo) was purchased from BiOSETTIA (San Diego, CA, USA).
Techniques: Clone Assay, Derivative Assay, Fractionation, Pull Down Assay, Activity Assay, Labeling